Status | green | Short name | 01. SP8x AOBS-WLL confocal | Full name | Leica SP8 AOBS-beamsplitting white-light laser confocal laser scanning microscope with in-line deconvolution | Description |
The SP8x confocal laser scanning microscope (Leica-microsystems) is mounted on a fully motorized DMi8 microscope on which x, y, z, t; lambda (wavelength), and sequential scans can be acquired at multiple positions and in z-stack. There are functions for stitching, and digital “best focus” determination also in z-stacks. Deconvolution can be done on the attached CUDA PC equipped with the LAS X module "Lightning". Live-cell imaging can be performed using a closed lid that controls CO2 and relative humidity levels, and using the big incubator box that controls temperature and airflow.
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Objectives
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10x / 0.40 Air (FWD = 2.2 mm)
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20x / 0.40 Air (7.5 mm long distance)
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40x / 0.60 Air (3.3 mm long distance)
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40x / 1.10 Water (0.65 mm)
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63x / 1.40 Oil (0.14 mm)
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100x / 1.40 Oil (0.13 mm)
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Lasers
405 nm diode laser + pulsed White Light Laser (WLL), up to 8 tunable excitation lines from 470-670nm can be applied simultaneously
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Detectors
2 GaAsP PMTs, 2 HyDs (high sensitivity, linear photon counting and time-gating), 1 normal transmitted-light PMT
In addition, the DMi8 microscope can be utilized for wide-field bright field and fluorescence observation. Images can be acquired with a monochrome DFC365FX camera..
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Filter cubes
DAPI (LP425), FITC (LP515), Rhod (LP590), and Y5 (BP 662-738)
| Category | GI- Light microscopy / Image analysis | Location | HG01.239 (optional AP1) | Access | Contact Jelle Postma | Applications | Confocal imaging (x,y,z,t,lambda, stitching, autofocus), deconvolution microscopy (resolution down to 140 nm), live cell imaging, photon counting and time-gating, flexibility of excitation (405 nm laser + WLL) and detection of emission. | Contact 1 | Jelle Postma j.postma@science.ru.nl Room: Huygens 01.222 | Contact 2 | Geert-Jan Janssen gja.Janssen@science.ru.nl Room: Huygens 01.222 | File 1 | Short instructions Sp8x (GI) | File 2 | Checklist settings sp8x | Bookings | Public booking info |
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