Microscope:
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Manual upright Olympus BX43F microscope
Excitation: Dual light sheet illumination through two 10x/0.25NA long distance Olympus objectives using a 4-laser combiner of the following laser lines:
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405nm/50mW
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488nm/50mW
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561nm/50mW
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638nm/100mW
Emission Selection: The system has the following filters mounted on a motorized filter wheel:
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ET440/40 nm
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ET525/50
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ET600/50
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ET690/50
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Notch filter 405/488/561/638
Detection: ORCA-FLASH4.0V3
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Scientific CMOS Sensor 2048 (H)×2048 (V), 6.5 μm×6.5 μm
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QE 82 % (@560 nm)
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Format 13.312 mm×13.312 mm
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Camera Link interface (100fps)
Objectives:
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10x/0.3 Water dipping long distance
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20x/0.5 Water dipping long distance
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40x/0.8 Water dipping long distance
Chamber and Sample holders: Chamber dimensions: width 21mm, length 70m height 25mm. Sample size up to 10 x 10 x 10 mm, cuvette filled with medium (volume < 15ml. Chamber highly resistant to corrosive medium: embryo media, salt water, clearing media. Agarose strips containing sampes can be mounted and observed straight away.
Motorized Rotation Module: Motorized rotation module with 1024 steps resolution, software controlled.
XY Tiling: XY motorized stage with 15mm range and 0.1um precision. Software controlled
Software: QtSPIM software for image acquisition XY, Z, channels, t (time), θ (rotation) and tiling
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