Microscope:
Automated DMi8 (inverted) with hardware autofocus (AFC) and motorized polarizer.
Excitation Lines: Lumencor 4-line LED with the following wavelengths
Emission Selection: EFW - external filter wheel slider for high speed acquisition. Filterset– quad cube for imaging (DAPI, FITC, TRITC, Cy5) including filter wheel for EFW
Detection: Leica DFC9000 GTC
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4.2 MP sCMOS camera, 6.5 μm pixel size (19 mm sensor diagonal)
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82% quantum efficiency, dark current of 0.14e-/p/sec
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~90 frames per second acquisition speed
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extended dynamic range of 33000:1
Objectives:
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20x/0.8 Air
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63x/1.2 Water
Stage: High speed/precision quantum stage
Environmental Control: Incubator with temperature controller
Software Modules:
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LAS X 3D for 3D rendering of acquired volumes
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“Navigator” for large tile scan
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“Thunder” for instant or volume computational clearing and improvement of resolution
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