Scientific Instruments Nijmegen

Categories - GI- Light microscopy / Image analysis

  3. Leica "Thunder"
Short name3. Leica "Thunder"
Full nameLeica DMi8 widefield microscope with "Thunder" fast computational clearing protocol


Automated DMi8 (inverted) with hardware autofocus (AFC) and motorized polarizer.

Excitation Lines: Lumencor 4-line LED with the following available wavelengths

  • 395 nm
  • 470 nm
  • 550 nm
  • 640 nm

Emission Selection:

DFT5 quad-cube for high speed fluorescence imaging (Blue, Green, Red, Far-red, or 100%) enabling specific capture of:

  • 420-450 nm (eg. DAPI, Hoechst)
  • 506-532 nm (eg. GFP, FITC)
  • 581-607 nm (eg. RFP, TRITC, Cy3)
  • 666-724 nm (eg. AlexaFluor 633, DRAQ5, Cy5)

Detection: Leica DFC9000 GTC

  • 4.2 MP sCMOS camera, 6.5 μm pixel size (19 mm sensor diagonal)
  • 82% quantum efficiency, dark current of 0.14e-/p/sec
  • ca. 90 frames per second acquisition speed
  • extended dynamic range of 33000:1


  • 5x/0.12 Dry
  • 10x/0.40 Dry
  • 20x/0.80 Air
  • 63x/1.20 Water

Stage: High speed/precision quantum stage

Environmental Control: Incubator with temperature controller

Software Modules:

  • LAS X 3D for 3D rendering of acquired volumes
  • “Navigator” for large tile scan
  • “Thunder” for instant or volume computational clearing and improvement of resolution
CategoryGI- Light microscopy / Image analysis
TechniqueWidefield fluorescence with computational clearing
AccessContact Jelle Postma
Contact 1Jelle Postma
Room: Huygens 01.222
Contact 2Geert-Jan Janssen
Room: Huygens 01.222
BookingsPublic booking info