Microscope:

Automated DMi8 (inverted) with hardware autofocus (AFC) and motorized polarizer.

Excitation Lines: Lumencor 4-line LED with the following wavelengths

• 395nm
• 488nm
• 561nm
• 638nm

Emission Selection: EFW - external filter wheel slider for high speed acquisition. Filterset– quad cube for imaging (DAPI, FITC, TRITC, Cy5) including filter wheel for EFW

Detection: Leica DFC9000 GTC

• 4.2 MP sCMOS camera, 6.5 μm pixel size (19 mm sensor diagonal)
• 82% quantum efficiency, dark current of 0.14e-/p/sec
• _~90 frames per second acquisition speed
• extended dynamic range of 33000:1

Objectives:

• 20x/0.8 Air
• 63x/1.2 Water

Stage: High speed/precision quantum stage

Environmental Control: Incubator with temperature controller

Software Modules:

• LAS X 3D for 3D rendering of acquired volumes
• “Navigator” for large tile scan
• “Thunder” for instant or volume computational clearing and improvement of resolution