Description |
The Leica DMIRE2 microscope is an inverted microcope equipped for bright field and fluorescence Wide-field imaging. A Photometrics CoolSnap K4 camera is attached. The camera, the filters turret, the zDrive, the XY stage and the transmitted light can be controlled by the uManager software.
Objectives: (Type, medium, Mag, NA, WD [mm], Max FOV [mm], catalog #)
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- HCPL fluotar Dry10x / 0.30 NA; 11.0 mm, maximum field of view 1.500 mm x 1.500 mm, 15506505
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- HC PL apo CS 20x / 0.70 D, also for DIC; 0.59 mm, maximum field of view 750 µm x 750 µm, 15506513
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- HCX apo Long working distance, Ultraviolet (VI) Water immersion 40x / 0.80 DIC; 3.3 mm,
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maximum field of view 375 µm x 375 µm, 15506155
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HCX PL apo CS 63x / 1.30 Glycerol immersion 21 C; 0.28 mm, maximum field of view 238.1 µm x 238.1 µm, 15506194
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- HCX Pl apo 63x / 1.40-0.60 (oil immersion with diaphragm) lambda blue-GFP; 0.10 mm,
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maximum field of view 238.1 µm x 238.1 µm, 15506192
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Fluorescence
Filter cube positions:
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A for UV excitation BP340-380, DM 400, LP 425
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N2.1 for green excitation BP 515-560, DM 580, LP 590
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I3 for blue excitation BP 450-490, DM 510, LP 515
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Scan for empty = transmission position
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BP = band pass, DM = Dichroic mirror, LP = Long pass
Light source; HBO mercury lamp (NOTICE: THE LAMPS SHOULD BE ALLOWED TO COOL DOWN BEFORE NEXT USE; RAPID OFF AND ON SWITCHING WILL HARM THE LAMP THAT MAY EVEN EXPLODE!)
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