Scientific Instruments Nijmegen
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Categories - GI- Light microscopy / Image analysis

  7. DMIRE2 inverted
Statusgreen
Short name7. DMIRE2 inverted
Full nameLeica DMIRE2 widefield fluorescence microscope
Description

The Leica DMIRE2 microscope is an inverted microcope equipped for bright field and fluorescence Wide-field imaging. A Photometrics CoolSnap K4 camera is attached. The camera, the filters turret, the zDrive, the XY stage and the transmitted light can be controlled by the uManager software.

Objectives: (Type, medium, Mag, NA, WD [mm], Max FOV [mm], catalog #)

  • - HCPL fluotar Dry10x / 0.30 NA; 11.0 mm, maximum field of view 1.500 mm x 1.500 mm, 15506505
  • - HC PL apo CS 20x / 0.70 D, also for DIC; 0.59 mm, maximum field of view 750 µm x 750 µm, 15506513
  • - HCX apo Long working distance, Ultraviolet (VI) Water immersion 40x / 0.80 DIC; 3.3 mm, 
  • maximum field of view 375 µm x 375 µm, 15506155
  • HCX PL apo CS 63x / 1.30 Glycerol immersion 21 C; 0.28 mm, maximum field of view 238.1 µm x 238.1 µm, 15506194
  • - HCX Pl apo 63x / 1.40-0.60 (oil immersion with diaphragm) lambda blue-GFP; 0.10 mm,
  • maximum field of view 238.1 µm x 238.1 µm, 15506192
  •  

Fluorescence 
Filter cube positions:

  • A for UV excitation BP340-380, DM 400, LP 425
  • N2.1 for green excitation BP 515-560, DM 580, LP 590
  • I3 for blue excitation BP 450-490, DM 510, LP 515
  • Scan for empty = transmission position
  • BP = band pass, DM = Dichroic mirror, LP = Long pass

Light source; HBO mercury lamp  (NOTICE: THE LAMPS SHOULD BE ALLOWED TO COOL DOWN BEFORE NEXT USE; RAPID OFF AND ON SWITCHING WILL HARM THE LAMP THAT MAY EVEN EXPLODE!)

CategoryGI- Light microscopy / Image analysis
LocationHG01.228
AccessContact Jelle Postma
ApplicationsTransmitted brightfield and fluorescence microscopy. Z-stacks, tile scans and time lapse acquisition.
Contact 1Jelle Postma
j.postma@science.ru.nl
Room: Huygens 01.222
BookingsPublic booking info