This JEOL FESEM can be used to make topographical images of the surface of objects, up to a resolution of about 1.5 nm.
It can be employed either in conventional SEM mode or as cryo-SEM.  In conventional use, the material needs to be naturally conductive or it has to be precoated with a thin layer of metal (use SEM coaters). In cryo-mode, the material is first fixed with nitrogen slush and transposed to the cryo-chamber. To observe inner structures, the frozen material can be cleaved, sublimated and coated with gold-palladium inside the vacuum cryo chamber.